Coding

Part:BBa_K3863007:Design

Designed by: LEONG WENG I   Group: iGEM21_PuiChing_Macau   (2021-09-26)


PHA-flag-PCT-HA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 25
    Illegal PstI site found at 118
    Illegal PstI site found at 208
    Illegal PstI site found at 280
    Illegal PstI site found at 742
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 25
    Illegal PstI site found at 118
    Illegal PstI site found at 208
    Illegal PstI site found at 280
    Illegal PstI site found at 742
    Illegal NotI site found at 1708
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1411
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 25
    Illegal PstI site found at 118
    Illegal PstI site found at 208
    Illegal PstI site found at 280
    Illegal PstI site found at 742
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 25
    Illegal PstI site found at 118
    Illegal PstI site found at 208
    Illegal PstI site found at 280
    Illegal PstI site found at 742
    Illegal NgoMIV site found at 1167
    Illegal NgoMIV site found at 1212
    Illegal AgeI site found at 448
    Illegal AgeI site found at 853
    Illegal AgeI site found at 1486
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 251


Design Notes

NA


Source

PHA Synthase (BBa_K1211002 from iGEM 2013 iGEM13_Yale) PCTcp (BBa_K1211001 from iGEM 2016 iGEM16_Evry)

References